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Updated: Jun 28, 2025
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Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
Published on: August 29, 2014
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Next-Generation Sequencing (NGS): Platforms and Applications
Jyoti S Mandlik1, Amol S Patil2, Sarita Singh1
1Department of Conservative Dentistry and Endodontics, Bharati Vidyapeeth (Deemed to be University) Dental College and Hospital, Pune, Maharashtra, India.
Journal of Pharmacy & Bioallied Sciences
|April 10, 2024
Summary
Next-generation sequencing (NGS) offers faster, cheaper DNA analysis for various applications. This technology comprehensively analyzes microbial species, including recent use in oral microbiome studies.
Area of Science:
- Genomics
- Molecular Biology
- Biotechnology
Background:
- Increasing demand for accurate, rapid, and cost-effective deoxyribonucleic acid (DNA) sequencing.
- Emergence of next-generation sequencing (NGS) technologies to meet these demands.
- NGS provides valuable insights for researchers and clinicians in developing treatment strategies.
Purpose of the Study:
- To highlight the broad applications of NGS in biology and medicine.
- To emphasize the capability of NGS in decoding life's complexities and improving life quality.
- To showcase the advantage of NGS over traditional methods for microbial identification.
Main Methods:
- Simultaneous sequencing of thousands to millions of molecules in parallel.
- Utilizing various NGS methodologies for comprehensive analysis.
- Application of NGS for oral microbial analysis.
Main Results:
- NGS enables faster and more inexpensive DNA sequencing.
- NGS facilitates comprehensive identification and characterization of microbial species.
- NGS is a powerful tool for improving crop quality and detecting pathogens.
Conclusions:
- NGS technologies are revolutionizing biological and medical research.
- NGS offers significant advantages over culture-based methods for microbial analysis.
- The application of NGS in oral microbiome analysis is a recent and promising development.
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