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Updated: Jun 28, 2025

Method for Labeling Transcripts in Individual Escherichia coli Cells for Single-molecule Fluorescence In Situ Hybridization Experiments
Published on: December 21, 2017
Fluorogenic cell surface glycan labelling with fluorescence molecular rotor dyes and nucleic acid stains
Alen Koçak1, Amal K Homer1, Antonia Feida1
1Department of Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Straße 2, 12489 Berlin, Germany. oliver.seitz@chemie.hu-berlin.de.
Abstract:
We show that covalent labelling of sialic acids on live cell surfaces or mucin increases the fluorescence of the fluorescence molecular rotors (FMRs) CCVJ, Cy3 and thioazole orange, enabling wash-free imaging of cell surfaces. Dual labelling with an FMR and an environmentally insensitive dye allows detection of changes that occur, for example, when cross-linking is altered.
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