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Updated: Jun 28, 2025

Determining Membrane Protein Topology Using Fluorescence Protease Protection FPP
Published on: April 20, 2015
Experimental and computational approaches for membrane protein insertion and topology determination
Gerard Duart1, Ricardo Graña-Montes1, Noelia Pastor-Cantizano1
1Departament de Bioquímica i Biologia Molecular, Institut Universitari de Biotecnologia i Biomedicina (BIOTECMED), Universitat de València, E-46100 Burjassot, Spain.
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Membrane proteins play pivotal roles in a wide array of cellular processes and constitute approximately a quarter of the protein-coding genes across all organisms. Despite their ubiquity and biological significance, our understanding of these proteins remains notably less comprehensive compared to their soluble counterparts. This disparity in knowledge can be attributed, in part, to the inherent challenges associated with employing specialized techniques for the investigation of membrane protein insertion and topology. This review will center on a discussion of molecular biology methodologies and computational prediction tools designed to elucidate the insertion and topology of helical membrane proteins.
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