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Simultaneous quantitation of 15 bioactive components in Yupingfeng granules by LC-MS/MS.

Le-Yi Huang1,2, Dao-Feng Chen1, Tong Wu2

  • 1School of Pharmacy, Fudan University, Shanghai, China.

Journal of Mass Spectrometry : JMS
|April 12, 2024
PubMed
Summary

Yupingfeng granules (YPFG) analysis is improved with a new liquid chromatography-tandem mass spectrometry (LC-MS/MS) method. This faster, simpler, and more accurate technique quantifies 15 bioactive compounds, aiding quality control.

Keywords:
LC–MS/MSYupingfeng granuleschromoneflavonesaponin

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Area of Science:

  • Pharmacology
  • Analytical Chemistry
  • Traditional Chinese Medicine

Background:

  • Yupingfeng granules (YPFG) are used for immunological, inflammatory, and pulmonary diseases.
  • Chromones, flavones, and saponins are key bioactive compounds in YPFG.
  • Accurate quantification methods for YPFG's bioactive compounds are lacking.

Purpose of the Study:

  • To establish a simple, rapid, and accurate liquid chromatography-tandem mass spectrometry (LC-MS/MS) method.
  • To quantify 15 major bioactive compounds in Yupingfeng granules (YPFG).
  • To provide a basis for YPFG quality standard improvement.

Main Methods:

  • Optimization of extraction parameters (solvent, time, ratio) and LC-MS/MS conditions.
  • Ultrasound-assisted extraction using 50% ethanol.
  • Chromatographic separation using a C18 column with formic acid/acetonitrile mobile phase.
  • Validation of linearity, precision, repeatability, stability, and recovery.

Main Results:

  • Optimal extraction: 0.1g YPFG, 50mL 50% ethanol, 30 min ultrasound.
  • The developed LC-MS/MS method demonstrated acceptable linearity, precision, repeatability, stability, and recovery.
  • Quantification of 15 bioactive compounds in seven YPFG batches.
  • Comparison with HPLC showed the LC-MS/MS method is faster, simpler, more accurate, and reliable.

Conclusions:

  • The established LC-MS/MS method is suitable for simultaneous determination of 15 compounds in YPFG.
  • This method offers significant advantages over existing HPLC techniques.
  • The findings support improved quality control and further research on YPFG's bioactive compounds.