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Updated: Jun 28, 2025
An Enrichment Method for Small Extracellular Vesicles Derived from Liver Cancer Tissue
Published on: February 3, 2023
Nanoquercetin and Extracellular Vesicles as Potential Anticancer Therapeutics in Hepatocellular Carcinoma
1Department of Anatomy and Cell Biology, College of Medicine, Gachon University, 155 Getbeol-ro, Yeonsu-gu, Incheon 21999, Republic of Korea.
Abstract:
Despite world-class sophisticated technologies, robotics, artificial intelligence, and machine learning approaches, cancer-associated mortalities and morbidities have shown continuous increments posing a healthcare burden. Drug-based interventions were associated with systemic toxicities and several limitations. Natural bioactive compounds derived nanoformulations, especially nanoquercetin (nQ), are alternative options to overcome drug-associated limitations. Moreover, the EVs-based cargo targeted delivery of nQ can have enormous potential in treating hepatocellular carcinoma (HCC). EVs-based nQ delivery synergistically regulates and dysregulates several pathways, including NF-κB, p53, JAK/STAT, MAPK, Wnt/β-catenin, and PI3K/AKT, along with PBX3/ERK1/2/CDK2, and miRNAs intonation. Furthermore, discoveries on possible checkpoints of anticancer signaling pathways were studied, which might lead to the development of modified EVs infused with nQ for the development of innovative treatments for HCC. In this work, we abridged the control of such signaling systems using a synergetic strategy with EVs and nQ. The governing roles of extracellular vesicles controlling the expression of miRNAs were investigated, particularly in relation to HCC.
Insights
Natural nanoquercetin delivered via extracellular vesicles shows promise for treating hepatocellular carcinoma by regulating key cancer pathways. This approach offers a potential alternative to conventional cancer therapies, reducing limitations and toxicities.
Area of Science:
- Biotechnology
- Nanomedicine
- Oncology
Background:
- Cancer mortality remains high despite advanced technologies.
- Conventional drug therapies for cancer have significant limitations and toxicities.
- Natural compounds like nanoquercetin offer potential alternative treatments.
Purpose of the Study:
- To investigate the potential of extracellular vesicle (EV)-based nanoquercetin (nQ) delivery for hepatocellular carcinoma (HCC) treatment.
- To explore the synergistic effects of EVs and nQ on cancer signaling pathways.
- To understand the role of EVs in regulating microRNAs (miRNAs) for HCC therapy.
Main Methods:
- Utilized extracellular vesicles (EVs) for targeted delivery of nanoquercetin (nQ).
- Investigated the modulation of key signaling pathways including NF-κB, p53, JAK/STAT, MAPK, Wnt/β-catenin, and PI3K/AKT.
- Analyzed the impact on PBX3/ERK1/2/CDK2 and miRNA expression in the context of HCC.
Main Results:
- EVs-based nQ delivery demonstrated synergistic regulation and dysregulation of multiple cancer-associated signaling pathways.
- Identified potential checkpoints within anticancer signaling pathways.
- Demonstrated the governing role of EVs in controlling miRNA expression relevant to HCC.
Conclusions:
- EVs-mediated targeted delivery of nQ presents a promising strategy for HCC treatment.
- This approach offers a synergistic effect by modulating critical signaling pathways and miRNAs.
- Further development of modified EVs with nQ could lead to innovative HCC therapies.
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