Related Experiment Video

Updated: Jun 28, 2025

Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding
10:13

Measuring G-protein-coupled Receptor Signaling via Radio-labeled GTP Binding

Published on: June 9, 2017

16.3K

Structure and function of an intermediate GPCR-Gαβγ complex.

Maxine Bi, Xudong Wang, Jinan Wang

    Biorxiv : the Preprint Server for Biology
    |April 15, 2024
    PubMed
    Summary

    Researchers uncovered a novel intermediate complex in G protein-coupled receptor (GPCR) signaling. This discovery reveals how GPCRs activate, aiding the development of targeted drugs for complex diseases.

    More Related Videos

    A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators07:41

    A Kinetic Fluorescence-based Ca2+ Mobilization Assay to Identify G Protein-coupled Receptor Agonists, Antagonists, and Allosteric Modulators

    Published on: February 20, 2018

    8.9K
    Strategic Screening and Characterization of the Visual GPCR-mini-G Protein Signaling Complex for Successful Crystallization09:19

    Strategic Screening and Characterization of the Visual GPCR-mini-G Protein Signaling Complex for Successful Crystallization

    Published on: March 16, 2020

    7.0K

    Related Concept Videos

    Activation and Inactivation of G Proteins01:22

    Activation and Inactivation of G Proteins

    Heterotrimeric G proteins are guanine nucleotide-binding proteins. As the name suggests, heterotrimeric G proteins are composed of three subunits: alpha, beta, and gamma. They remain GDP-bound or GTP-bound inside the cells and switch between inactive/active states. The Gα subunit possesses the nucleotide-binding pocket that binds guanine nucleotides and switches between GDP or GTP-bound states. In contrast, the Gꞵ and Gγ subunits are always bound together with high...
    7.1K
    G-protein Coupled Receptors01:21

    G-protein Coupled Receptors

    G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.
    118.6K
    Transducer Mechanism: G Protein–Coupled Receptors01:30

    Transducer Mechanism: G Protein–Coupled Receptors

    G Protein–Coupled Receptors (GPCRs) are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to various stimuli. GPCRs regulate critical physiological pathways and are excellent drug targets for treating diseases such as diabetes, cancer, obesity, depression, or Alzheimer's. Nearly 35% of approved drugs implement their therapeutic effects by selectively interacting with specific GPCRs.
    GPCRs are also called heptahelical,...
    2.0K
    G Protein-coupled Receptors01:15

    G Protein-coupled Receptors

    G Protein-Coupled Receptors or GPCRs are membrane-bound receptors that transiently associate with heterotrimeric G proteins and induce an appropriate response to sensory stimuli such as light, odors, hormones, cytokines, or neurotransmitters.
    GPCRs are also called heptahelical, 7TM, or serpentine receptors, and consist of seven (H1-H7) transmembrane alpha-helices that span the bilayer to form a cylindrical core. The transmembrane helices are connected by three extracellular loops and three...
    11.9K
    Assembly of Signaling Complexes01:30

    Assembly of Signaling Complexes

    Multiprotein signaling complexes are formed in a dynamic process involving protein-protein interactions at the cytoplasmic domain of transmembrane receptors or enzymatic and non-enzymatic proteins associated with the receptor. These complexes ensure the activation and propagation of intracellular signals that regulate cell functions.
    Interaction domains in cell signaling
    Interaction domains recognize exposed features of their binding partners containing post-translationally modified sequences,...
    5.8K
    GPCR Desensitization01:12

    GPCR Desensitization

    G protein-coupled receptor (GPCR) signaling plays a crucial role in cell functioning. GPCR desensitization is an equally essential process. It allows cells to respond to changing environments and regain sensitivity to new stimuli while preventing unnecessary stimulation when no longer needed. Prolonged exposure to stimuli leads to GPCR desensitization. It involves blocking the receptors from binding and activating additional G proteins. This inhibits activation of downstream effectors, thereby...
    6.0K