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Analysis of human monocyte chemoattractant binding by flow cytometry.
Journal of Leukocyte Biology
|September 1, 1985
Summary
Human monocytes have receptors for the chemotactic peptide fMet-Leu-Phe-Lys, but not all respond. While 60% bind the peptide, only 36% migrate, indicating a subset binds but doesn't move.
Area of Science:
- Immunology
- Cell Biology
Background:
- The chemotactic peptide fMet-Leu-Phe-Lys attracts human blood monocytes.
- Only a third of monocytes typically respond to this chemoattractant.
Purpose of the Study:
- To investigate if non-responding monocytes lack attractant receptors.
- To analyze the binding of fMet-Leu-Phe-Lys to human monocytes.
Main Methods:
- Peripheral blood mononuclear cells were incubated with fMet-Leu-Phe-Lys-FITC.
- Peptide binding was quantified using flow cytometry at 0°C.
- Monocyte migration was assessed in response to the attractant.
Main Results:
- The fluoresceinated peptide demonstrated rapid binding and saturation with increasing concentration.
- Approximately 60% of blood monocytes exhibited binding of the peptide.
- In contrast, only 36% of monocytes showed directed migration.
Conclusions:
- A significant proportion of monocytes (approximately 40%) do not bind the attractant.
- A subset of monocytes binds the peptide but fails to exhibit directed migration.
- This suggests a dissociation between receptor binding and functional chemotaxis in some monocytes.