Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.0K
Overview of Electron Microscopy01:25

Overview of Electron Microscopy

9.1K
The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.
9.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Journal of microscopy·2024
Same author

Structured illumination ophthalmoscope: super-resolution microscopy on the living human eye.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2022
Same author

Spatially modulated illumination microscopy: application perspectives in nuclear nanostructure analysis.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2022
Same author

Characterization of brain-derived extracellular vesicles reveals changes in cellular origin after stroke and enrichment of the prion protein with a potential role in cellular uptake.

Journal of extracellular vesicles·2020
Same author

A novel assay for exosomal and cell-free miRNA isolation and quantification.

RNA biology·2020
Same author

Sample drift estimation method based on speckle patterns formed by backscattered laser light.

Biomedical optics express·2019
Same journal

In operando imaging of the space-charge region in a 4H-SiC MOSCAP using STEM-EBIC.

Journal of microscopy·2026
Same journal

The future of DXA: How AI is transforming bone health diagnostics.

Journal of microscopy·2026
Same journal

The Origins of Ploem's Filter Cube: A Pandora's Box.

Journal of microscopy·2026
Same journal

The reproducibility gap in graph neural network workflows for cell dynamics: A checklist-driven case study.

Journal of microscopy·2026
Same journal

Assessing the reproducibility of a bioimage analysis workflow characterising tissue flow in Drosophila.

Journal of microscopy·2026
Same journal

Modular training resources for bioimage analysis.

Journal of microscopy·2026
See all related articles

Related Experiment Video

Updated: Jun 28, 2025

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis
14:53

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

Published on: February 3, 2018

7.1K

Modulated illumination microscopy: Application perspectives in nuclear nanostructure analysis.

Christoph Cremer1,2,3, Florian Schock1, Antonio Virgilio Failla4

  • 1Kirchhoff Institute for Physics (KIP), Heidelberg, Germany.

Journal of Microscopy
|April 15, 2024
PubMed
Summary
This summary is machine-generated.

Modulated Illumination Microscopy (MIM) offers advanced insights into cell nucleus structures. Combining MIM with single-molecule localization microscopy (SMLM) achieves nanoscale precision for analyzing macromolecular complexes.

Keywords:
nuclear genome structurestructured illumination microscopysuper‐resolution microscopy

More Related Videos

Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules
09:45

Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules

Published on: August 8, 2019

10.0K
Single-Molecule Imaging of Nuclear Transport
12:13

Single-Molecule Imaging of Nuclear Transport

Published on: June 9, 2010

13.4K

Related Experiment Videos

Last Updated: Jun 28, 2025

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis
14:53

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

Published on: February 3, 2018

7.1K
Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules
09:45

Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules

Published on: August 8, 2019

10.0K
Single-Molecule Imaging of Nuclear Transport
12:13

Single-Molecule Imaging of Nuclear Transport

Published on: June 9, 2010

13.4K

Area of Science:

  • Cell Biology
  • Advanced Light Microscopy
  • Nanoscale Imaging

Background:

  • The cell nucleus structure in higher organisms is a key area of advanced light microscopy research.
  • Existing methods include confocal, 4Pi, STED, localization microscopy, and patterned illumination microscopy (lateral or axial modulation).

Purpose of the Study:

  • To discuss application perspectives of Modulated Illumination Microscopy (MIM) and its combination with single-molecule localization microscopy (SMLM).
  • To highlight the potential for nanoscale analysis of macromolecular nuclear complexes.

Main Methods:

  • Spatially Modulated Illumination Microscopy (SMI) for axial dimension determination and positioning of fluorescent objects.
  • Combination of axially modulated SMI with laterally structured illumination (SIM).
  • Utilizing fluorescence yields typical for SMLM applications.

Main Results:

  • SMI precisely determines the axial size (40-200 nm diameter) of isolated fluorescent objects with nanometer precision.
  • SMI enables axial positioning of structures down to the 1 nm scale.
  • The combined SMI/SIM approach is expected to yield 3D localization precision below 1 nm.

Conclusions:

  • The combination of MIM techniques, particularly SMI and SIM, offers unprecedented resolution for nuclear structure analysis.
  • This approach provides nanoscale sizing and positioning capabilities, approaching cryo-electron microscopy resolution.
  • It enables detailed analysis of macromolecular nuclear complexes.