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Analytical Methods for Brassinosteroid Analysis: Recent Advances and Applications

Jana Oklestkova1, Miroslav Kvasnica1, Miroslav Strnad1

  • 1Laboratory of Growth Regulators, Faculty of Science, Palacký University & Institute of Experimental Botany, Czech Academy of Sciences, Šlechtitelů 27, Olomouc CZ-78371, Czech Republic.

Plant & Cell Physiology
|April 15, 2024
PubMed

Keywords:
BrassinosteroidsChemical synthesisChromatographyImmunoassaysMass spectrometryQuantification

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For solutions containing mixtures of different cations, the identity of each cation can be determined by qualitative analysis. This technique involves a series of selective precipitations with different chemical reagents, each reaction producing a characteristic precipitate for a specific group of cations. Metal ions within a group are further separated by varying the pH, heating the mixture to redissolve a precipitate, or adding other reagents to form complex ions.
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Electrodeposition is a technique used to separate an analyte from interferents by electrochemical processes. Here, the analyte is a metal ion that can be deposited on an electrode immersed in the sample solution. The electrochemical setup consists of an anode and a cathode. When an electric current is applied to the setup, oxidation occurs at the anode. At the cathode, which consists of a large metal surface, metal ions undergo reduction and deposit onto the surface.
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