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Updated: Jun 28, 2025
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Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
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Free Radical Lipid Peroxidation Induced by Reactive Halogen Species
Oleg M Panasenko1, Yury A Vladimirov2, Valery I Sergienko2
1Lopukhin Federal Research and Clinical Center of Physical-Chemical Medicine, Federal Medical Biological Agency, Moscow, Russia. o-panas@mail.ru.
Biochemistry. Biokhimiia
|April 15, 2024
Summary
Reactive halogen species (RHS), produced by myeloperoxidase (MPO), initiate and propagate lipid peroxidation (LPO) chain reactions. This process contributes to various human inflammatory diseases.
Area of Science:
- Biochemistry
- Oxidative Stress
- Enzymology
Background:
- Reactive halogen species (RHS) are generated by heme peroxidases like myeloperoxidase (MPO) in mammals.
- Lipid peroxidation (LPO) is a key process in oxidative stress and cellular damage.
- RHS involvement in LPO mechanisms is not fully elucidated.
Purpose of the Study:
- To review the mechanisms of RHS-initiated free radical lipid peroxidation (LPO).
- To explore the dual role of RHS in LPO initiation and propagation.
- To discuss the implications of RHS-induced LPO in human inflammatory diseases.
Main Methods:
- Literature review of biochemical and enzymatic studies.
- Analysis of reaction pathways involving RHS, LPO, and biological molecules.
- Correlation of RHS-induced LPO with disease pathogenesis.
Main Results:
- RHS participate in both initiation and branching steps of LPO chain reactions.
- Free radical formation occurs via RHS reactions with nitrite and amino groups.
- RHS react with lipid hydroperoxides, generating peroxyl and alkoxyl radicals.
Conclusions:
- RHS-induced LPO is a significant contributor to oxidative stress.
- Understanding these mechanisms is crucial for inflammatory disease research.
- RHS-mediated LPO plays a role in cardiovascular, neurodegenerative, and other inflammatory conditions.
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