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Related Concept Videos

Methods of Sterilization I: Physical Methods01:29

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As used in a healthcare facility, sterilization destroys all microorganisms through physical or chemical methods. The physical method includes steam, dry heat, boiling water, and radiation.
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
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Cleaning, disinfection, and sterilization are the methods that help to break the infection chain and prevent disease.
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In healthcare, the chemical method of sterilization uses chemical sterilants to treat surgical instruments and medical supplies to help prevent the transmission of infectious pathogens to patients. Due to heat sensitivity, most medical supplies and equipment should not be exposed to high temperatures. These parts include rubber, plastic, glass, and other similar elements.
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Updated: Jun 28, 2025

Treating Surfaces with a Cold Atmospheric Pressure Plasma using the COST-Jet
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Cold Plasma: Clean Technology to Destroy Pathogenic Micro-organisms.

R Kar1, N Chand1, A Bute1,2

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Transactions of the Indian National Academy of Engineering : an International Journal of Engineering and Technology
|April 16, 2024
PubMed
Summary
This summary is machine-generated.

Atmospheric pressure cold plasma effectively kills pathogenic microorganisms. Modified devices show promise for disinfecting areas, including potential applications for COVID-19 wards without harsh chemicals.

Keywords:
Cold plasmaGermicidal efficiencyOptical emission spectroscopy

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Area of Science:

  • Plasma physics
  • Microbiology
  • Public health

Background:

  • Pathogenic microorganisms pose significant health risks.
  • Cold plasma is an emerging technology for microbial inactivation.
  • The COVID-19 pandemic highlighted the need for effective disinfection methods.

Purpose of the Study:

  • To evaluate the efficacy of modified atmospheric pressure cold plasma devices against pathogenic microorganisms.
  • To compare the performance of two distinct cold plasma device designs.
  • To investigate the potential application of cold plasma for disinfecting large areas, such as hospital wards.

Main Methods:

  • Modification of two cold plasma devices: a pencil-like microwave-based device and a larger radiofrequency (RF) plasma-based device.
  • Testing microbial inactivation efficacy against *Aeromonas* bacteria and its bacteriophage.
  • Utilizing Optical Emission Spectroscopy (OES) to analyze plasma characteristics, specifically the presence of hydroxyl (OH) radicals.
  • Assessing the impact of distance and treatment time on microbial killing efficiency.

Main Results:

  • Both modified cold plasma devices demonstrated significant efficiency in killing *Aeromonas* bacteria and its bacteriophage.
  • The microwave-based device inactivated microbes within 2 minutes at a 6 cm distance.
  • The larger RF plasma device achieved similar microbial inactivation over a larger area (approx. 40 cm²) within 4 minutes.
  • Optical Emission Spectroscopy confirmed the production of OH radicals by both devices, correlating with microbial destruction.

Conclusions:

  • Atmospheric pressure cold plasma is a viable technology for inactivating pathogenic microorganisms.
  • Modified cold plasma devices, particularly the larger RF-based system, offer effective and efficient microbial disinfection capabilities.
  • These findings suggest potential applications for cold plasma in decontaminating large spaces, including hospital environments affected by pathogens like SARS-CoV-2, without chemical residues.