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Cleaning, Sterilization, and Disinfection01:30

Cleaning, Sterilization, and Disinfection

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Cleaning, disinfection, and sterilization are the methods that help to break the infection chain and prevent disease.
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The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
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Methods of Sterilization II: Chemical Methods01:30

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In healthcare, the chemical method of sterilization uses chemical sterilants to treat surgical instruments and medical supplies to help prevent the transmission of infectious pathogens to patients. Due to heat sensitivity, most medical supplies and equipment should not be exposed to high temperatures. These parts include rubber, plastic, glass, and other similar elements.
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Methods of Sterilization I: Physical Methods01:29

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As used in a healthcare facility, sterilization destroys all microorganisms through physical or chemical methods. The physical method includes steam, dry heat, boiling water, and radiation.
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
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Hand hygiene01:23

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Asepsis is the practice of preventing or breaking the chain of infection. The nurse employs aseptic techniques to prevent the spread of microorganisms and reduce the risk of diseases. Hand hygiene is the cornerstone of aseptic techniques and is classified into medical and surgical asepsis. Medical asepsis includes hand hygiene and the use of gloves. Surgical asepsis, or the sterile technique, refers to practices that render and keep objects and areas free of microorganisms.
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Related Experiment Video

Updated: Jun 28, 2025

The Portable Chemical Sterilizer PCS, D-FENS, and D-FEND ALL: Novel Chlorine Dioxide Decontamination Technologies for the Military
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Fight Against COVID-19: ARCI's Technologies for Disinfection.

B V Sarada1, R Vijay1, R Johnson1

  • 1International Advanced Research Centre for Powder Metallurgy and New Materials (ARCI), Balapur (PO), Hyderabad, Telangana 500005 India.

Transactions of the Indian National Academy of Engineering : an International Journal of Engineering and Technology
|April 16, 2024
PubMed
Summary

Researchers developed rapid disinfection systems to combat the spread of COVID-19 (SARS-CoV-2). These systems utilize UVC germicidal lamps, dry heat, and chemical disinfectants for effective microbial inactivation.

Keywords:
Disinfection systemsHOCLHeat sterilizationSARS-CoV-2UVC radiation

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Area of Science:

  • Microbiology
  • Public Health
  • Biomedical Engineering

Background:

  • The global population faces significant concern due to the highly contagious nature of COVID-19, caused by the SARS-CoV-2 virus.
  • Effective control of viral spread is a primary focus in combating the pandemic.

Purpose of the Study:

  • To develop and validate rapid and effective disinfection systems for inactivating SARS-CoV-2 and other pandemic-causing microorganisms.

Main Methods:

  • Development of a UVC-based disinfection trolley.
  • Implementation of a honeycomb air heater.
  • Design of a fogging chamber utilizing UVC germicidal lamps, dry heat sterilization, and hypochlorous acid (HOCl)-based chemical disinfectant.

Main Results:

  • The developed disinfection systems demonstrated rapid and effective inactivation of microorganisms.
  • Successful deployment and validation of these systems in various hospital settings.

Conclusions:

  • The ARCI-developed disinfection technologies offer a viable solution for rapid microbial inactivation.
  • These systems are crucial for controlling the spread of pandemic-causing agents in healthcare environments.