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CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

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Updated: Jun 28, 2025

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

Deconstructing cancer with precision genome editing.

Grace A Johnson^1,2, Samuel I Gould^1,2, Francisco J Sánchez-Rivera^1,2

¹Department of Biology, Massachusetts Institute of Technology, Cambridge 02142, MA, U.S.A.

Biochemical Society Transactions

|April 17, 2024

Summary

This summary is machine-generated.

Precision genome editing tools like base editing and prime editing enable precise study of cancer mutations. These technologies offer versatile and scalable methods for advancing cancer research and future therapeutic strategies.

Keywords:

CRISPR functional genomics genetics genome editing genomics mouse models

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Area of Science:

Genomics
Molecular Biology
Cancer Research

Background:

Genome editing technologies enable precise engineering of genetic mutations.
Base editing and prime editing are emerging as leading tools in genome engineering.
These techniques allow for the study of cancer-associated mutations in their native genomic environments.

Purpose of the Study:

To review the advantages and disadvantages of base editing and prime editing.
To discuss the application of these precision genome editing tools in contemporary cancer research.
To explore potential future directions and integration of these technologies in the field.

Main Methods:

Review of current literature on base editing and prime editing.
Analysis of the applications of these technologies in cancer mutation studies.
Speculative discussion on future integration and advancements.

Main Results:

Base editing and prime editing offer high precision and efficiency for genetic engineering.
These methods are versatile and scalable, making them valuable for research.
The review highlights their current utility and potential in cancer research.

Conclusions:

Base editing and prime editing are powerful tools for understanding cancer genetics.
Further integration of these technologies could accelerate cancer research and therapeutic development.
Continued advancements in precision genome editing hold significant promise for oncology.