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Updated: Jun 28, 2025
A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices
Published on: July 26, 2011
Two-Photon FRET/FLIM Imaging of Cerebral Neurons
Thomas T Luyben1,2, Jayant Rai1,2, Bingyue Zhou1,2
1Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, Canada.
Abstract:
Two-photon FRET (Förster resonance energy transfer) and FLIM (fluorescence lifetime imaging microscopy) enable the detection of FRET changes of fluorescence reporters in deep brain tissues, which provide a valuable approach for monitoring target molecular dynamics and functions. Here, we describe two-photon FRET and FLIM imaging techniques that allow us to visualize endogenous and optogenetically induced cAMP dynamics in living neurons with genetically engineered FRET-based cAMP reporters.
