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Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...

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Related Experiment Video

Updated: May 8, 2026

Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells

Generation of Standardized and Reproducible Forebrain-type Cerebral Organoids from Human Induced Pluripotent Stem Cells

Published on: January 23, 2018

Generating Homogeneous Brain Organoids from Human iPSCs.

Xianwei Chen¹, Yanhong Shi²

¹Department of Neurodegenerative Diseases, Beckman Research Institute of City of Hope, Duarte, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|April 17, 2024

Summary

Developing consistent, high-yield human brain organoids is crucial for studying neurological diseases. This new method uses large-scale embryoid body generation and quality control to improve brain organoid production for research.

Keywords:

Alzheimer’s disease Brain organoids Differentiation Disease modeling Embryoid bodies (EBs)Induced pluripotent stem cells (iPSCs)Neurological diseases Pluripotent stem cells (PSCs)

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Last Updated: May 8, 2026

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Published on: January 23, 2018

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Published on: March 4, 2020

Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors

Brain Organoid Generation from Induced Pluripotent Stem Cells in Home-Made Mini Bioreactors

Published on: December 11, 2021

Area of Science:

Neuroscience
Stem Cell Biology
Developmental Biology

Background:

Human brain tissues are inaccessible for research, necessitating advanced in vitro models.
Human induced pluripotent stem cell (hiPSC)-derived brain organoids offer a promising model for studying brain development and diseases.
Current challenges include generating a large quantity of consistent brain organoids for disease modeling.

Purpose of the Study:

To describe a novel method for generating high-yield, consistent brain organoids.
To facilitate the study of human brain development and neurological disorders using improved in vitro models.

Main Methods:

Combines large-scale embryoid body (EB) generation.
Incorporates a quality control screening step during differentiation.
Focuses on robust generation of brain organoids from hiPSCs.

Main Results:

Achieved high-yield production of brain organoids.
Demonstrated high consistency in the generated brain organoids.
Established a robust method for brain organoid generation.

Conclusions:

The described method provides a reliable approach for generating brain organoids.
This advancement supports the study of human brain development and neurological disease modeling.
Enables more consistent and scalable research into brain disorders.