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Updated: Jun 28, 2025
In utero Electroporation followed by Primary Neuronal Culture for Studying Gene Function in Subset of Cortical Neurons
Published on: October 8, 2010
Brain Sample Preparation After Performing in Utero Electroporation to Proliferating and Differentiated Cells in the
Minkyung Shin1, Yoichi Kosodo2,3
1Korea Brain Research Institute (KBRI), Daegu, Republic of Korea.
None:
In utero electroporation (IUE) enables labeling and manipulating specific types of cells by introducing DNA plasmids with desired promoters. After the surgery, mouse brains are fixed at any stage and analyzed after staining using specific antibodies. Here, we describe the flow of the IUE experiment from the preparation to microscopic observations.
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