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Related Experiment Video

Updated: Jun 28, 2025

High-Throughput DNA Plasmid Multiplexing and Transfection Using Acoustic Nanodispensing Technology
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Acoustothermal transfection for cell therapy.

Xiufang Liu1, Ning Rong1, Zhenhua Tian2

  • 1CAS Key Laboratory of Health Informatics, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, 1068 Xueyuan Avenue, Shenzhen 518055, China.

Science Advances
|April 17, 2024
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Summary
This summary is machine-generated.

A novel acoustothermal transfection method safely and efficiently delivers genetic material into stem cells and T cells, overcoming limitations of current techniques for cell therapy and immunotherapy. This advance promises improved cellular and gene therapies.

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Regenerative Medicine

Background:

  • Transfection is crucial for cell therapy and immunotherapy but faces efficiency-viability trade-offs.
  • Current methods struggle to balance high transfection rates with cell survival.

Purpose of the Study:

  • To develop a novel transfection method balancing efficiency and cell viability.
  • To demonstrate the efficacy of acoustothermal transfection for primary T cells and stem cells.

Main Methods:

  • Utilized acoustothermal effects to enhance cell membrane and nuclear envelope permeability.
  • Applied the method to primary T cells and mesenchymal stem cells (MSCs).
  • Delivered two plasmid types simultaneously into MSC nuclei.

Main Results:

  • Achieved high transfection efficiencies of 89.6 ± 1.2% in MSCs.
  • Demonstrated successful in vivo targeting of cerebral ischemia sites with CXCR4-transfected MSCs.
  • Showed reduced infarct volume in mice following treatment.

Conclusions:

  • The acoustothermal method overcomes key limitations in transfection efficiency and cell viability.
  • This technique offers a powerful new tool for cellular and gene therapies.
  • Potential applications include personalized cell therapy and immunotherapy.