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Related Concept Videos

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Related Experiment Video

Updated: Jun 28, 2025

Isolation of Adult Human Dermal Fibroblasts from Abdominal Skin and Generation of Induced Pluripotent Stem Cells Using a Non-Integrating Method
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Reprogramming fibroblast into human iBlastoids.

Jia Ping Tan1,2,3, Xiaodong Liu4,5,6,7, Jose M Polo8,9,10,11,12

  • 1Department of Anatomy and Developmental Biology, Monash University, Clayton, Victoria, Australia.

Nature Protocols
|April 17, 2024
PubMed
Summary
This summary is machine-generated.

Researchers developed induced blastoids (iBlastoids), an in vitro human blastocyst model, using somatic reprogramming. This new model offers a more complex system for studying early human development compared to current methods.

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Related Experiment Videos

Last Updated: Jun 28, 2025

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Area of Science:

  • Developmental Biology
  • Stem Cell Biology
  • Reproductive Medicine

Background:

  • Current models for early human embryogenesis, including donated blastocysts and simplified stem cell cultures, have limitations in capturing developmental complexity.
  • Pluripotent and trophoblast stem cells have been valuable but lack the spatiotemporal and molecular dynamics of early embryonic development.

Purpose of the Study:

  • To describe a protocol for generating induced blastoids (iBlastoids), an in vitro integrated model of the human blastocyst.
  • To provide an alternative and more complex in vitro model for deciphering human embryogenesis.

Main Methods:

  • Somatic reprogramming of human dermal fibroblasts to generate reprogramming intermediates.
  • Subsequent generation of iBlastoids from these intermediates, a process taking approximately 27 days.
  • Discussion of characterization and functional assays for iBlastoids.

Main Results:

  • Successful generation of iBlastoids, an in vitro model mimicking the human blastocyst.
  • A detailed protocol is provided, requiring approximately one year of experience in cell culture and reprogramming assays.
  • iBlastoids offer an alternative tool for studying early human development.

Conclusions:

  • Induced blastoids (iBlastoids) provide a novel and more complex in vitro model for studying early human embryogenesis.
  • This protocol facilitates the scientific community's exploration of human development.
  • iBlastoids serve as a valuable alternative to traditional blastocyst models in research.