Multi-Sensor Origami Platform: A Customizable System for Obtaining Spatiotemporally Precise Functional Readouts in 3D...

Area of Science:

• Biomedical Engineering
• Tissue Engineering
• Biofabrication

Background:

• 3D bioprinting allows in vitro tissue models with native morphology and function.
• Obtaining functional readouts from bioprinted tissues is challenging due to sensor placement difficulties.
• Directly bioprinting onto sensors interferes with printer heads.

Purpose of the Study:

• To introduce a multi-sensing origami platform (MSOP) for improved functional assessment of 3D bioprinted tissues.
• To enable precise electrode insertion into pre-fabricated 3D tissue constructs.
• To overcome limitations of current sensor integration in bioprinting.

Main Methods:

• Developed an origami-inspired platform that folds around 3D tissue structures.
• Utilized computer-aided-design for custom electrode placement.
• Integrated the MSOP with a commercial multi-electrode array (MEA) system.
• Demonstrated electrode integration for recording neuronal activity and measuring endothelial barrier function.

Main Results:

• Successfully recorded neuronal electrical activity in a 3D neurovascular unit model using integrated 3D MEA electrodes.
• Measured endothelial barrier function via impedance-based sensors within the MSOP.
• Showcased the platform's versatility by measuring neuronal activity in brain organoids.

Conclusions:

• The MSOP facilitates precise, non-invasive sensor integration in 3D bioprinted models.
• This platform enhances functional characterization of complex tissue constructs.
• The MSOP holds promise for advancing in vitro tissue modeling and drug screening.