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Affinity Chromatography

Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...

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Related Experiment Video

Updated: Jun 28, 2025

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library

Published on: January 14, 2020

High-Throughput DNA-Encoded Libraries Affinity Selection Platform for Binder Identification with Solid Support

Ramón Rama-Garda^1,2, Eduardo Domínguez³, María Isabel Loza²

¹Discovery Chemistry Research & Technologies, Lilly Research Laboratories, Eli Lilly and Company, Alcobendas, Spain.

Assay and Drug Development Technologies

|April 19, 2024

Summary

This summary is machine-generated.

A new automated platform enhances DNA-encoded library (DEL) screening for ligand identification. This high-throughput system improves reproducibility and flexibility in affinity selection (AS) for drug discovery research.

Keywords:

DNA-encoded libraries affinity selection automation quality control

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Primer-Free Aptamer Selection Using A Random DNA Library

Primer-Free Aptamer Selection Using A Random DNA Library

Published on: July 26, 2010

Area of Science:

Biochemistry
Chemical Biology
Drug Discovery

Background:

DNA-encoded libraries (DELs) are powerful tools for identifying novel ligands.
Affinity selection (AS) is the core method used in DELs to find binders.
Current AS methods can face challenges in reproducibility and throughput.

Purpose of the Study:

To develop a high-throughput, automated affinity selection (AS) platform for DNA-encoded libraries (DELs).
To enhance the reproducibility and flexibility of AS processes in ligand identification.
To create a platform compatible with industrial standards for DEL applications.

Main Methods:

Development of a fully automated platform for high-throughput affinity selection.
Implementation of two primary protein immobilization techniques: magnetic beads and resin tip columns.
Configurable protocol options supporting diverse DEL types and experimental conditions for 96 simultaneous samples.

Main Results:

The platform demonstrates high throughput and flexibility, accommodating various DELs and AS methods.
It supports both magnetic beads and resin tip column immobilization for proteins.
The system allows for routine DEL screens, covalent library screening, and protein immobilization validation.

Conclusions:

The automated AS platform significantly improves the reproducibility of DEL binder identification.
Its versatility makes it suitable for a wide range of applications in academic and industrial drug discovery.
This technology offers a powerful instrument for advancing ligand identification using DELs.