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LINC00998 Modulating M2 Macrophage Activation in Allergic Rhinitis by Stabilizing BOB.1 mRNA.

Yan He1, Yulei Tang1, Silu Wen1

  • 1Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei, People's Republic of China.

Journal of Inflammation Research
|April 19, 2024
PubMed
Summary

This study reveals that the long noncoding RNA LINC00998 regulates macrophage polarization in allergic rhinitis (AR). Lower LINC00998 levels promote M2 macrophage polarization, offering new insights into AR pathogenesis.

Keywords:
LINC00998LncRNAallergic rhinitisallergymacrophage polarization

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Area of Science:

  • Immunology
  • Molecular Biology
  • Genetics

Background:

  • Allergic rhinitis (AR) presents a growing global health challenge with significant socioeconomic impact.
  • Long noncoding RNAs (lncRNAs) are increasingly recognized for their role in allergic responses.
  • This study investigates the specific involvement of lncRNA LINC00998 in the mechanisms underlying AR.

Purpose of the Study:

  • To explore the role of lncRNA LINC00998 in the pathogenesis of allergic rhinitis (AR).
  • To investigate the relationship between LINC00998 expression and macrophage polarization in AR.
  • To elucidate the molecular mechanism by which LINC00998 influences macrophage polarization.

Main Methods:

  • Assessed LINC00998 expression in peripheral blood mononuclear cells from AR patients using qRT-PCR.
  • Utilized in vitro models (THP-1 cells, primary human macrophages) and an AR mouse model to study macrophage polarization.
  • Employed gain- and loss-of-function experiments, qRT-PCR, flow cytometry, and Western blot to analyze LINC00998's mechanism.

Main Results:

  • LINC00998 expression was significantly decreased in AR patients and M2 macrophages.
  • Suppression of LINC00998 enhanced M2 polarization, while overexpression attenuated it.
  • LINC00998 regulated BOB.1 expression, which in turn modulated macrophage polarization via the PU.1/IL-1β axis.

Conclusions:

  • The lncRNA LINC00998 plays a crucial role in modulating M2 macrophage polarization in allergic rhinitis.
  • LINC00998 exerts its effects by regulating the BOB.1/PU.1/IL-1β signaling pathway.
  • These findings provide novel insights into AR pathogenesis and potential therapeutic targets.