Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.4K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparing Liquid Vortex Capture & the Rapid Droplet Sampling Interface for Single Cell Mass Spectrometry.

Journal of the American Society for Mass Spectrometry·2026
Same author

One Step Block-like Copolymers from an Upcycled Monomer Using Ring-Opening Metathesis Polymerization.

ACS polymers Au·2026
Same author

Rapid Generation of Tandem Mass Spectrometry Reference Libraries Using Immediate Drop-on-Demand Liquid Handling Coupled to an Open Port Sampling Interface.

Journal of the American Society for Mass Spectrometry·2026
Same author

Correction: HunStat2 - a simple and low-cost potentiostat with electrochemical impedance spectroscopy capability.

Analytical methods : advancing methods and applications·2026
Same author

HunStat2 - a simple and low-cost potentiostat with electrochemical impedance spectroscopy capability.

Analytical methods : advancing methods and applications·2025
Same author

Evaluating inkjet dispenser/liquid vortex capture-mass spectrometry for single-cell metabolomics in Hep G2 steatosis caused by tamoxifen.

Analytical and bioanalytical chemistry·2025

Related Experiment Video

Updated: Jun 28, 2025

Shotgun Lipidomics of Rodent Tissues
11:46

Shotgun Lipidomics of Rodent Tissues

Published on: November 18, 2022

2.0K

Improved lipid analysis using a 2D-LC-MS system with a novel injection procedure.

József Pánczél1, Vilmos Kertesz2, Matthias Schiell1

  • 1Research and Development, DMPK, Sanofi-Aventis Deutschland GmbH, Frankfurt am Main, Germany.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|April 19, 2024
PubMed
Summary
This summary is machine-generated.

This study enhances nonpolar lipidomics analysis using reversed-phase chromatography. A novel sample preparation and injection method significantly improves lipid detection sensitivity and peak shape for better lipid quantification.

Keywords:
LipidomicsMass spectrometryReversed phase (RP) chromatographySignal enhancement

More Related Videos

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
09:26

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Published on: November 17, 2011

16.1K
Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

6.9K

Related Experiment Videos

Last Updated: Jun 28, 2025

Shotgun Lipidomics of Rodent Tissues
11:46

Shotgun Lipidomics of Rodent Tissues

Published on: November 18, 2022

2.0K
Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis
09:26

Cellular Lipid Extraction for Targeted Stable Isotope Dilution Liquid Chromatography-Mass Spectrometry Analysis

Published on: November 17, 2011

16.1K
Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization
12:11

Simultaneous Affinity Enrichment of Two Post-Translational Modifications for Quantification and Site Localization

Published on: February 27, 2020

6.9K

Area of Science:

  • Lipidomics
  • Chromatography
  • Analytical Chemistry

Background:

  • Nonpolar lipid analysis presents challenges in sample extraction and chromatography.
  • Existing methods often suffer from analyte loss, peak distortion, and limited sensitivity.

Purpose of the Study:

  • To develop an improved method for analyzing nonpolar lipidomics sample extracts using reversed-phase (RP) chromatography.
  • To enhance sensitivity and peak quality for both targeted and untargeted lipid quantification.

Main Methods:

  • A 4:3:3 (v/v/v) methanol/methyl tert-butyl ether/chloroform (MMC) mixture was used for sample extraction.
  • The LC system was flushed with methanol to prevent carryover and analyte loss.
  • A large sample volume (100 μl) was injected after a fivefold dilution with methanol, followed by online aqueous eluent for analyte focusing.

Main Results:

  • The method achieved quantitative transfer of 25 lipid classes to the column head.
  • Methanol's weak elution strength prevented peak distortions, resulting in narrow peaks (FWHM 0.07-0.08 min).
  • Enhanced sensitivity was observed, with limits of detection typically in the sub-nanomolar region.

Conclusions:

  • The developed RP chromatography method significantly improves nonpolar lipid analysis.
  • The approach enables precise targeted lipid quantification and reliable untargeted quantitative comparisons.
  • This method offers enhanced sensitivity and peak resolution for comprehensive lipidomics studies.