Recombinant study of the mouse molar cervical loop and dental papilla by renal transplantation

Cervical loop tissues of the mandibular first molar germs were dissected from 18-day mouse embryos and recombined with the isochronal dental papilla mesenchyme. The recombinants were transplanted under the kidney capsule of syngeneic male mice. Five days later, proliferation of epithelial and mesenchymal cells was observed. At the junctional region between the internal dental epithelium and the dental papilla mesenchyme, extracellular organic matrix was deposited. By 10 days after, transplantation, the formation of dentine and enamel was initiated. Twenty days after transplantation, the recombinant developed into an almost intact molar tooth. The compensatory potential of the molar cervical loop, when recombined with the dental papilla mesenchyme, was indicated.