Three-dimensional hotspot structures constructed from nanoporous gold with a V-cavity and gold nanoparticles for...

The intensity and sensitivity of surface-enhanced Raman scattering (SERS) spectra are highly dependent on the consistency and homogeneity of the nanomaterials. In this study, we developed a large-area three-dimensional (3D) hotspot substrate with good homogeneity and reproducibility in SERS signals. The substrate is based on the synergistic structures of nanoporous gold (NPG) and gold nanoparticles (AuNPs). NPG was combined with a periodic V-shaped nanocavity array to create nanoporous gold with a V-cavity (NPGVC) array featuring uniform hotspots. A nanoporous gold V-shaped resonant cavity (NPGVRC) structure was developed by incorporating AuNPs into the NPGVC array. The coupling action between the AuNPs and NPGVC resulted in a SERS-enhanced electromagnetic field with 3D hotspot distribution. The strategic incorporation of NPG and V-cavity array significantly expanded the surface area available for analyte adsorption and interaction with AuNPs. Using rhodamine 6G (R6G) and malachite green (MG) as probe molecules, the SERS performance was investigated, and the NPGVRC substrate not only showed excellent enhancement with the limit of detection as low as 10^-11 M, but also presented good homogeneity. NPGVRC was then used for biological detection of the influenza A virus, where we acquired and examined the characteristic SERS spectra of two spike proteins. It is demonstrated that there is significant potential for our proposed SERS platform to be used in biosensors.