Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Electrospray Ionization (ESI) Mass Spectrometry

Electrospray Ionization (ESI) Mass Spectrometry

Higher molecular weight biomolecules are nonvolatile compounds that may decompose before ionizing or vaporizing during mass analysis with conventional electron impact ionization methods. Accordingly, electrospray ionization (ESI) is the favored method for vaporizing and ionizing biomolecules as it circumvents rapid fragmentation and enables the recording of mass signals for the entire biomolecule.
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A Combined Omics Approach to Elucidate the Molecular Interplay behind a Beneficial <i>Arabidopsis-Caulobacter</i> Interaction.

Journal of proteome research·2026

Same author

Proteomic profiling of whole tissue sections in cardiac ATTR amyloidosis reveals increased extracellular matrix remodeling.

Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology·2026

Same author

Pneumococcus uses COMMD2 to alter host cellular immunity.

Nature communications·2026

Same author

A Platform for High-throughput and Ultrasensitive Immunopeptidomics.

Molecular & cellular proteomics : MCP·2026

Same author

Identification of DksA as a novel pro-inflammatory mediator of <i>Pseudomonas aeruginosa</i> under conditions mimicking chronic cystic fibrosis lung infection.

Virulence·2026

Same author

Single-cell proteomics reveals cytoplasmic defects in Patl2-deficient oocytes rescued by spindle transfer.

Human reproduction (Oxford, England)·2026

Same journal

Biodegradable Self-Powered Electrotherapy Patch for Integrated Smart Wound Management.

Analytical chemistry·2026

Same journal

Metabolite Fraction Libraries for Quantitative NMR Metabolomics.

Analytical chemistry·2026

Same journal

Self-Contained Lateral-Flow Microfluidic Bead-Based Assay for Rapid Quantification of Early-Stage Kidney Biomarkers.

Analytical chemistry·2026

Same journal

Overcoming the Debye Shielding Effect with Concave-Convex Structures for Sensitivity-Enhanced Thin-Film Transistors.

Analytical chemistry·2026

Same journal

Mode-Phase-Difference Photothermal Spectroscopy Assisted by a Bent Biconically Tapered Microfiber for Gas Sensing.

Analytical chemistry·2026

Same journal

Negative-Pressure-Actuated Microfluidics: A Dual-Mode Point-of-Care Sensor for Allergen-Specific IgE in Interstitial Fluid.

Analytical chemistry·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 16, 2026

Digital Microfluidics for Automated Proteomic Processing

Digital Microfluidics for Automated Proteomic Processing

Published on: November 6, 2009

High-Throughput Nanoflow Proteomics Using a Dual-Column Electrospray Source.

An Staes^1,2,3, Katie Boucher^1,2,3, Sara Dufour^1,2,3

¹VIB Center for Medical Biotechnology, Technologiepark-Zwijnaarde 75, B9052 Ghent, Belgium.

Analytical Chemistry

|April 22, 2024

Summary

This summary is machine-generated.

Dual-column nano-LC-MS enhances proteomic analysis throughput. A two-outlet electrospray source setup doubles throughput without sacrificing sensitivity, crucial for clinical and single-cell proteomics.

More Related Videos

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes

Published on: August 6, 2018

Related Experiment Videos

Last Updated: Jun 16, 2026

Digital Microfluidics for Automated Proteomic Processing

Digital Microfluidics for Automated Proteomic Processing

Published on: November 6, 2009

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Published on: November 15, 2017

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes

2 in 1: One-step Affinity Purification for the Parallel Analysis of Protein-Protein and Protein-Metabolite Complexes

Published on: August 6, 2018

Area of Science:

Proteomics
Analytical Chemistry
Mass Spectrometry

Background:

Clinical and single-cell proteomics demand high throughput and sensitivity.
Advancements in mass spectrometry (MS) increase sensitivity, enabling shorter liquid chromatography (LC) methods.
Maximizing analytical throughput is essential for processing large sample numbers.

Purpose of the Study:

To evaluate a dual-column nano-LC-MS setup with a two-outlet electrospray source.
To compare its performance against a classic dual-column setup with a single-outlet source.
To determine if throughput can be doubled without compromising sensitivity.

Main Methods:

Implementation of a dual-column nano-LC-MS system.
Utilizing a two-outlet electrospray ionization source.
Comparison of analytical performance with a single-outlet source configuration.

Main Results:

The dual-column setup with a two-outlet source demonstrated doubled throughput.
Sensitivity was maintained, allowing for deep proteome analysis.
This configuration is effective for handling low-input samples.

Conclusions:

A dual-column nano-LC-MS setup with a two-outlet electrospray source effectively doubles analytical throughput.
This approach maintains high sensitivity, vital for clinical and single-cell proteomics.
The optimized configuration enhances the efficiency of proteomic analyses.