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DNA Isolation

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...

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Related Experiment Video

Updated: Jun 28, 2025

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Published on: March 9, 2015

Cleaning protocols in forensic genetic laboratories.

Marie-Louise Kampmann¹, Jacob Tfelt-Hansen^2,3, Claus Børsting²

¹Section of Forensic Genetics, Department of Forensic Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark. marie.louise.kampmann@sund.ku.dk.

International Journal of Legal Medicine

|April 22, 2024

Summary

This summary is machine-generated.

Effective DNA removal in forensic labs is crucial. Freshly made household bleach and Virkon® successfully eliminated amplifiable DNA from surfaces, unlike other tested reagents.

Keywords:

Contamination DNA Forensic genetics Laboratory cleaning PCR qPCR

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Area of Science:

Forensic Science
Molecular Biology
Laboratory Safety

Background:

Cross-sample contamination poses a significant risk in forensic genetic analyses.
Standardized DNA removal protocols are essential for maintaining laboratory integrity.
Existing cleaning protocols in forensic laboratories vary widely.

Purpose of the Study:

To evaluate the efficacy of different cleaning protocols used in forensic genetic laboratories.
To identify optimal reagents for removing amplifiable DNA from laboratory surfaces.
To compare the DNA removal efficiency of various disinfection agents.

Main Methods:

A survey of ten forensic genetic laboratories was conducted to collect information on their cleaning protocols.
The efficiency of different cleaning reagents and protocols was tested on various surfaces.
Amplifiable DNA levels were quantified after surface cleaning to assess reagent effectiveness.

Main Results:

Household bleach (freshly made) and Virkon® demonstrated complete removal of amplifiable DNA from surfaces.
DNA AWAY™, ethanol, isopropanol, and ChemGene HLD4L failed to eliminate all amplifiable DNA.
Variability in cleaning reagent selection was observed among participating laboratories.

Conclusions:

Freshly prepared household bleach and Virkon® are highly effective for DNA decontamination in forensic settings.
Certain common laboratory disinfectants are insufficient for complete removal of amplifiable DNA.
Standardization of effective DNA cleaning protocols is recommended for forensic genetic laboratories.