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Updated: Jun 28, 2025

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
Published on: May 20, 2013
Spectral Reflectance Measurements
John Stamford1, Piotr Kasznicki1, Tracy Lawson2
1School of Life Sciences, University of Essex, Colchester, UK.
Abstract:
This chapter provides a methodology for evaluating plant health and leaf characteristics using spectral reflectance. It provides a step-by-step guide to using spectrometers for high-resolution point measurements of leaf spectral reflectance and multispectral imaging for capturing spatial data, emphasizing the importance of consistent measurement conditions. The chapter further explores the intricacies of multispectral imaging, including calibration, data collection, and image processing. Finally, this chapter delves into the application of various spectral indices for the quantification of key traits such as pigment content, the status of the xanthophyll cycle, water content, and how to identify spectral regions of interest for further research and development. Serving as a guide for researchers and practitioners in plant science, this chapter provides a straightforward framework for plant health assessment using spectral reflectance.
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