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Updated: Jun 28, 2025
Isolation of Exosomes from the Plasma of HIV-1 Positive Individuals
Published on: January 5, 2016
A protocol for anti-CD4 IgG antibody purification using plasma samples from people with HIV and antibody-mediated
Zhenwu Luo1, Wei Jiang1,2,3
1Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC, USA.
Background:
Up to 20% of people with HIV (PWH) fail to recover their CD4+ T cell counts to levels similar to healthy controls after suppressive antiretroviral therapy (ART). Immune non-responders (INRs) are PWH on suppressive ART with CD4+ T cell counts lower than 350 cells/mL, whereas their CD8+ T cell counts are higher than healthy controls. We are the first group to report that increased anti-CD4 autoantibody IgGs in INRs are responsible for blunted CD4+ T cell reconstitution in PWH with ART and viral suppression through anti-CD4 IgG-induced antibody-mediated cytotoxicity (ADCC) against CD4+ T cells in vitro. Notably, anti-CD4 IgG-mediated poor CD4+ T cell recovery from suppressive ART is the only mechanism targeting CD4+ T cells, specifically.
Results:
We provide a detailed one-by-one step protocol from antigen-specific antibody isolation using plasma samples, to ADCC assay.
Conclusions:
To promote reproducible research, a detailed protocol for isolating anti-CD4 IgG autoantibodies from plasma samples of PWH and evaluating ADCC effects is reported here.•Antigen-specific antibody isolation using human plasma samples•Antibody-mediated cytotoxicity (ADCC).
