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Updated: Jun 28, 2025

Efficient and Site-specific Antibody Labeling by Strain-promoted Azide-alkyne Cycloaddition
Published on: December 23, 2016
Ultrafast Au(III)-Mediated Arylation of Cysteine
Evan A Doud1, James A R Tilden2, Joseph W Treacy1,3
1Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California 90095, United States.
Abstract:
Through mechanistic work and rational design, we have developed the fastest organometallic abiotic Cys bioconjugation. As a result, the developed organometallic Au(III) bioconjugation reagents enable selective labeling of Cys moieties down to picomolar concentrations and allow for the rapid construction of complex heterostructures from peptides, proteins, and oligonucleotides. This work showcases how organometallic chemistry can be interfaced with biomolecules and lead to a range of reactivities that are largely unmatched by classical organic chemistry tools.
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