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Updated: Apr 12, 2026
Detection of Protein Interactions in Plant using a Gateway Compatible Bimolecular Fluorescence Complementation BiFC System
Published on: September 16, 2011
Protein-Protein Interactions Visualized by Bimolecular Fluorescence Complementation in Arabidopsis thaliana
Aswathy Jayasree1, Hymavathi Salava2, Tomasz Nodzynski1
1Central European Institute of Technology (CEITEC), Masaryk University, Brno, Czech Republic.
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Bimolecular fluorescence complementation (BiFC) is a powerful tool for studying protein-protein interactions in living cells. By fusing interacting proteins to fluorescent protein fragments, BiFC allows visualization of spatial localization patterns of protein complexes. This method has been adapted to a variety of expression systems in different organisms and is widely used to study protein interactions in plant cells. The Agrobacterium-mediated transient expression protocol for BiFC assays in Nicotiana benthamiana (N. benthamiana) leaf cells is widely used, but in this chapter, a method for BiFC assay using Arabidopsis thaliana protoplasts is presented.
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