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Conformational Evaluation of HIV-1 Trimeric Envelope Glycoproteins Using a Cell-based ELISA Assay
Published on: September 14, 2014
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B cell immunofocusing and repriming in two HIV-1 Env immunization regimens
Jenna M DeLuca1, Maria Blasi2,3, Shalini Jha2,4
1Translational Immunobiology Unit, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA.
Research Square
|April 25, 2024
Summary
Diverse viruses challenge vaccine design. This study shows different HIV-1 Env immunization strategies elicit distinct memory B-cell responses, with one strategy repriming the B-cell response due to immunogen format changes.
Area of Science:
- Immunology
- Virology
- Vaccine Development
Background:
- Developing effective vaccines against rapidly mutating viruses like HIV-1 is challenging.
- Understanding B-cell responses to different immunogen designs is crucial for optimizing vaccine strategies.
Purpose of the Study:
- To evaluate memory B-cell cross-reactivity against HIV-1 vaccine components in non-human primates (NHPs).
- To compare the impact of two distinct multivalent immunization strategies on B-cell responses.
Main Methods:
- Analysis of memory B-cell responses from two independent NHP trials.
- Assessment of cross-reactivity with specific HIV-1 Env vaccine components.
- Comparison of responses following multiclade Env immunization versus sequential gp140 Env immunization (non-stabilized followed by SOSIP-stabilized trimers).
Main Results:
- A multiclade, multivalent HIV-1 Env immunization regimen induced a dominant memory B-cell response targeting shared epitopes.
- Sequential immunization with different formats of HIV-1 Envs (gp140 non-stabilized followed by SOSIP-stabilized trimers) led to a repriming of the B-cell response.
- The change in immunogen format significantly altered the B-cell response trajectory.
Conclusions:
- Immunization strategy significantly influences the resulting memory B-cell repertoire and epitope targeting.
- Sequential immunization with different immunogen formats can re-engage and redirect B-cell responses.
- These findings have implications for designing more effective HIV-1 vaccines by tailoring immunogen sequences and formats.
