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Area of Science:

  • Supramolecular chemistry
  • Biomimetic chemistry

Background:

  • Foldamers can form tertiary helix-turn-helix structures via hydrogen bonding.
  • Creating abiotic quaternary structures from these tertiary folds is a key challenge.

Purpose of the Study:

  • To design and synthesize novel foldamer sequences capable of forming abiotic quaternary structures.
  • To investigate the self-assembly behavior of these engineered foldamers.

Main Methods:

  • Solid-state X-ray crystallography
  • Solution-state Nuclear Magnetic Resonance (NMR) spectroscopy
  • Circular Dichroism (CD) spectroscopy

Main Results:

  • Engineered foldamers did not form quaternary structures as intended.
  • Instead, stable domain-swapped dimer motifs were observed.
  • Structural analysis revealed a reorganization of hydrogen bonds and altered helix orientation and handedness.

Conclusions:

  • The designed foldamers self-assemble into domain-swapped dimers, not quaternary structures.
  • Domain swapping represents a significant structural reorganization pathway for these foldamers.
  • Understanding this process provides insights into abiotic self-assembly and protein-like structural motifs.