Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

TREM1 signaling amplifies neutrophil-mediated inflammation in inflammatory bowel disease.

Nature communications·2026
Same author

Combined antagonism of Oncostatin M (OSM) and Interleukin 6 (IL-6) provides both anti-fibrotic and anti-inflammatory benefit in pulmonary fibrosis.

bioRxiv : the preprint server for biology·2026
Same author

IL-27 elicits a cytotoxic CD8<sup>+</sup> T cell program to enforce tumour control.

Nature·2025
Same author

i-shaped antibody engineering enables conformational tuning of biotherapeutic receptor agonists.

Nature communications·2024
Same author

Antibody homotypic interactions are encoded by germline light chain complementarity determining region 2.

Proceedings of the National Academy of Sciences of the United States of America·2022
Same author

Oncostatin M expression induced by bacterial triggers drives airway inflammatory and mucus secretion in severe asthma.

Science translational medicine·2022

Related Experiment Video

Updated: Jun 27, 2025

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker
08:12

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker

Published on: January 3, 2019

7.3K

AviTrap: A novel solution to achieve complete biotinylation.

Cheng Lu1,2, Jack Bevers2, Tulika Tyagi2

  • 1Department of Protein Sciences, Genentech Inc., South San Francisco, CA, United States of America.

Plos One
|April 25, 2024
PubMed
Summary

Purifying biotinylated proteins is challenging due to incomplete reactions. A new antibody resin, AviTrap, efficiently isolates biotinylated AviTagged proteins by capturing non-biotinylated byproducts in a single step.

More Related Videos

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions
08:14

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

Published on: July 17, 2019

12.6K
Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses
11:19

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses

Published on: February 25, 2011

19.9K

Related Experiment Videos

Last Updated: Jun 27, 2025

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker
08:12

Single-step Purification of Macromolecular Complexes Using RNA Attached to Biotin and a Photo-cleavable Linker

Published on: January 3, 2019

7.3K
In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions
08:14

In Vitro Biochemical Assays using Biotin Labels to Study Protein-Nucleic Acid Interactions

Published on: July 17, 2019

12.6K
Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses
11:19

Isolation of Translating Ribosomes Containing Peptidyl-tRNAs for Functional and Structural Analyses

Published on: February 25, 2011

19.9K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Protein Chemistry

Background:

  • Site-specific protein labeling using AviTag and BirA enzyme is common.
  • Incomplete biotinylation reactions yield mixtures of biotinylated and non-biotinylated proteins.
  • Existing methods lack efficient purification strategies for biotinylated proteins.

Purpose of the Study:

  • To develop an efficient purification method for biotinylated AviTagged proteins.
  • To overcome challenges associated with incomplete biotinylation reactions.
  • To provide a solution for enriching specifically labeled proteins.

Main Methods:

  • Development of a monoclonal antibody targeting the non-biotinylated AviTag sequence.
  • Conjugation of the antibody to a resin to create AviTrap.
  • One-step purification of biotinylated AviTagged proteins using AviTrap.

Main Results:

  • The developed monoclonal antibody specifically recognizes non-biotinylated AviTag.
  • AviTrap effectively traps non-biotinylated AviTagged proteins.
  • Fully biotinylated proteins are efficiently separated and pass through the resin.

Conclusions:

  • AviTrap offers a rapid and efficient one-step purification method for biotinylated AviTagged proteins.
  • This technology simplifies the enrichment of specifically labeled proteins.
  • It addresses the limitations of current protein labeling and purification techniques.