Related Experiment Video
Updated: Jun 27, 2025
09:43
Hand-held Clinical Photoacoustic Imaging System for Real-time Non-invasive Small Animal Imaging
Published on: October 16, 2017
11.5K
Xanthene-Based Dyes for Photoacoustic Imaging and their Use as Analyte-Responsive Probes
Frederik Brøndsted1, Cliff I Stains1,2,3
1Department of Chemistry, University of Virginia, 22904, Charlottesville, VA, USA.
Chemistry (Weinheim an Der Bergstrasse, Germany)
|April 25, 2024
Summary
Developing advanced photoacoustic imaging (PAI) probes using modified xanthene dyes allows for in vivo detection of various disease biomarkers. These near-infrared absorbing probes enhance PAI capabilities for diagnostics and research.
Area of Science:
- Biomedical Imaging
- Chemical Biology
- Organic Chemistry
Background:
- Photoacoustic imaging (PAI) offers high-resolution, deep-tissue visualization with diagnostic potential.
- Near-infrared (NIR) absorbing small-molecule dyes are crucial for advancing PAI capabilities.
- Activity-based sensing (ABS) using chemoselective dyes enables analyte detection.
Purpose of the Study:
- To present strategies for red-shifting xanthene dye spectral properties.
- To explore the development of analyte-responsive PAI probes based on xanthenes.
- To demonstrate in vivo detection of specific disease-relevant analytes using these probes.
Main Methods:
- Modification of xanthene scaffolds via heteroatom or auxochrome additions to red-shift NIR absorption.
- Application of chemoselective ring-opening strategies for probe activation.
- In vivo PAI for detecting hypochlorous acid, nitric oxide, copper (II), NQO1, and carbon monoxide.
Main Results:
- Successful red-shifting of xanthene spectral properties was achieved.
- Developed PAI probes demonstrated analyte-responsive behavior.
- In vivo detection of multiple disease-relevant analytes was successfully performed using xanthene-based ABS probes.
Conclusions:
- Xanthene dyes are versatile scaffolds for developing advanced PAI probes.
- Strategies for spectral tuning and chemoselective sensing are effective.
- These probes hold significant promise for in vivo disease diagnostics and research.
