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PRRX1 upregulates PD-L1 in human mesenchymal stem cells

Taro Osawa1, Daisuke Yamada1, Tomoko Takao1

  • 1Department of Regenerative Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8558, Japan.

In Vitro Cellular & Developmental Biology. Animal
|April 25, 2024
PubMed

Insights

Paired-related homeobox (PRRX1) enhances Programmed death-ligand 1 (PD-L1) expression in mesenchymal stem cells (MSCs). This finding suggests PRRX1 may regulate MSC immunomodulation via the PD-L1 pathway for treating immune disorders.

Area of Science:

  • Immunology
  • Stem Cell Biology
  • Molecular Biology

Background:

  • Mesenchymal stem cells (MSCs) show promise in treating immune disorders like graft-versus-host disease (GvHD) and Crohn's disease.
  • Programmed death-ligand 1 (PD-L1) on MSCs plays a crucial immunosuppressive role, but its regulation is not fully understood.

Purpose of the Study:

  • To investigate the role of paired-related homeobox (PRRX1) in regulating PD-L1 expression in MSCs.
  • To explore PRRX1's influence on MSC surface antigen expression and immunomodulatory potential.

Main Methods:

  • Analysis of PRRX1 isoform effects on MSC marker gene expression (CD26, CD317).
  • Quantification of PD-L1 mRNA and protein levels following PRRX1 manipulation.

Main Results:

  • All tested PRRX1 isoforms increased mRNA levels of MSC markers CD26 and CD317.
  • PRRX1 isoforms significantly elevated both mRNA and protein expression of PD-L1 in MSCs.

Conclusions:

  • PRRX1 positively regulates PD-L1 expression in MSCs.
  • PRRX1 may function as an immunomodulatory factor in MSCs by influencing the PD-L1 pathway.
Keywords:
ImmunomodulationMesenchymal stem cellsPaired-related homeobox 1Programmed death-ligand 1

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