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PRRX1 upregulates PD-L1 in human mesenchymal stem cells
Taro Osawa1, Daisuke Yamada1, Tomoko Takao1
1Department of Regenerative Science, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama, 700-8558, Japan.
In Vitro Cellular & Developmental Biology. Animal
|April 25, 2024
Summary
Paired-related homeobox (PRRX1) enhances Programmed death-ligand 1 (PD-L1) expression in mesenchymal stem cells (MSCs). This finding suggests PRRX1 may regulate MSC immunomodulation via the PD-L1 pathway for treating immune disorders.
Area of Science:
- Immunology
- Stem Cell Biology
- Molecular Biology
Background:
- Mesenchymal stem cells (MSCs) show promise in treating immune disorders like graft-versus-host disease (GvHD) and Crohn's disease.
- Programmed death-ligand 1 (PD-L1) on MSCs plays a crucial immunosuppressive role, but its regulation is not fully understood.
Purpose of the Study:
- To investigate the role of paired-related homeobox (PRRX1) in regulating PD-L1 expression in MSCs.
- To explore PRRX1's influence on MSC surface antigen expression and immunomodulatory potential.
Main Methods:
- Analysis of PRRX1 isoform effects on MSC marker gene expression (CD26, CD317).
- Quantification of PD-L1 mRNA and protein levels following PRRX1 manipulation.
Main Results:
- All tested PRRX1 isoforms increased mRNA levels of MSC markers CD26 and CD317.
- PRRX1 isoforms significantly elevated both mRNA and protein expression of PD-L1 in MSCs.
Conclusions:
- PRRX1 positively regulates PD-L1 expression in MSCs.
- PRRX1 may function as an immunomodulatory factor in MSCs by influencing the PD-L1 pathway.
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