Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

The Supercomplexes in the Crista Membrane01:41

The Supercomplexes in the Crista Membrane

2.5K
The mitochondrial cristae membrane is the primary site for the oxidative phosphorylation (OXPHOS) process of energy conversion mediated through respiratory complexes I to V. These complexes have been widely studied for decades, and it has been proven that they form supramolecular structures called respiratory supercomplexes (SC). These higher-order complexes may be crucial in maintaining the biochemical structure and improving the physiological activity of the individual complexes while...
2.5K
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

3.3K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.3K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.0K
Protein Complex Assembly02:41

Protein Complex Assembly

10.6K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
10.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Morphology and Phase Engineering of TaS<sub>2</sub> Nanosheets on Planar Au Substrates Toward Superior Electrocatalytic Property.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Space-Confined Chemical Vapor Deposition of 2D FeS With Crossover Magnetoresistance and Ultra-High Conductivity.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Integrative Multi-Omics Analysis Reveals Nutritional Metabolite Diversity and Regulatory Mechanisms in <i>Ocimum basilicum</i>.

Life (Basel, Switzerland)·2026
Same author

A novel ABCD1 frameshift mutation detected in a Chinese male with adrenomyeloneuropathy.

Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology·2026
Same author

Discovering multiscale deep formulas in complex systems via neural-guided lambda calculus.

Nature communications·2026
Same author

The investigation on tensile failure of active waveguide structure by acoustic emission measurements.

Scientific reports·2026
Same journal

DeepDOX1: A Dual-Drive Framework Integrating Deep Learning and First-Principles Quantum Chemistry for Drug-Protein Affinity Prediction.

JACS Au·2026
Same journal

Catalyst-Controlled Regiodivergent C-H Olefination of Furanyl Carbamates through a Rational Approach.

JACS Au·2026
Same journal

Charting the Biosynthetic Landscape of Hybrid Polyketide-Nonribosomal Peptide-Specialized Lipids.

JACS Au·2026
Same journal

Valence-State-Dependent Surface Lattice Oxygen in CeO<sub>2</sub>‑Modified VPO Catalysts: Elucidating the Mechanism of <i>n</i>‑Butane Selective Oxidation to Maleic Anhydride.

JACS Au·2026
Same journal

Quantitative Insights into Pressure-Dependent Mass Transport and Reaction Kinetics in Electrochemical CO<sub>2</sub> Reduction.

JACS Au·2026
Same journal

3‑Methylthiopropionic Acid Kills Carbapenem-Resistant <i>Klebsiella pneumoniae</i> by Disrupting Membrane Integrity and Bioenergetics.

JACS Au·2026
See all related articles

Related Experiment Video

Updated: Jun 27, 2025

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy
11:26

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

Published on: September 8, 2009

9.3K

Understanding Emergent Complexity from a Single-Molecule Perspective.

Yilin Guo1, Mingyao Li2, Cong Zhao3

  • 1Beijing National Laboratory for Molecular Sciences, National Biomedical Imaging Center, College of Chemistry and Molecular Engineering, Peking University, 292 Chengfu Road, Haidian District, Beijing 100871, P. R. China.

JACS Au
|April 26, 2024
PubMed
Summary
This summary is machine-generated.

Investigating molecular interactions using single-molecule junctions reveals how complex behaviors emerge from simple components. This approach offers a bottom-up strategy for understanding emergent properties in complex systems.

More Related Videos

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
09:30

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Published on: August 6, 2018

9.4K
Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells
06:48

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Published on: January 5, 2024

3.5K

Related Experiment Videos

Last Updated: Jun 27, 2025

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy
11:26

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

Published on: September 8, 2009

9.3K
Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy
09:30

Analyzing Dynamic Protein Complexes Assembled On and Released From Biolayer Interferometry Biosensor Using Mass Spectrometry and Electron Microscopy

Published on: August 6, 2018

9.4K
Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells
06:48

Author Spotlight: Evaluation of Protein-Condensate Dynamics in Live Human Cells

Published on: January 5, 2024

3.5K

Area of Science:

  • Molecular interactions
  • Complex systems science
  • Single-molecule electronics

Background:

  • Complex behaviors in systems arise from diverse molecular structures and interactions.
  • Understanding emergent properties requires studying individual components and their interactions.
  • Single-molecule junctions offer a platform to probe these fundamental molecular behaviors.

Purpose of the Study:

  • To highlight the role of single-molecule junctions in observing molecular interactions.
  • To explore how interactions at the single-molecule level contribute to emergent complexity.
  • To outline future directions for single-molecule junctions in complex system research.

Main Methods:

  • Utilizing electrical signals from single-molecule junctions.
  • Observing individual molecular behaviors and interactions.
  • Investigating intramolecular orbital, intermolecular, and chemical reaction interactions.

Main Results:

  • Single-molecule junctions enable direct observation of molecular behaviors and interactions.
  • Diverse interactions, including orbital and weak intermolecular forces, can be studied.
  • This technique provides a foundation for bottom-up studies of emergent complexity.

Conclusions:

  • Single-molecule junctions are powerful tools for dissecting emergent complexity.
  • Understanding molecular interactions is key to understanding complex systems.
  • Future research can leverage single-molecule junctions to explore more complex emergent phenomena.