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Updated: May 5, 2026

Isolation of Specific Neuron Populations from Roundworm Caenorhabditis elegans

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Published on: August 6, 2019

RNA Isolation from Ctenophores.

Andrea B Kohn¹, Yelena Bobkova¹, Leonid L Moroz^2,3

¹Whitney Laboratory for Marine Bioscience, University of Florida, St. Augustine, FL, USA.

Methods in Molecular Biology (Clifton, N.J.)

|April 26, 2024

Summary

This summary is machine-generated.

This study presents a cost-effective and reproducible RNA isolation protocol for RNA sequencing (RNA-seq) in ctenophores. The method efficiently analyzes low-quantity samples from various developmental stages and tissues.

Keywords:

Ctenophora Development Pleurobrachia RNA-seq Single cells Transcriptome

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Area of Science:

Marine Biology
Genomics
Developmental Biology

Background:

Transcriptome analysis using RNA sequencing (RNA-seq) is crucial in biomedical research.
Specific RNA isolation methods are needed for non-model organisms like ctenophores.
Analyzing small cell populations and individual cells requires sensitive techniques.

Purpose of the Study:

To develop and present an efficient RNA isolation protocol for ctenophores.
To enable RNA-seq library preparation from low-quantity samples.
To validate the protocol across different ctenophore species and developmental stages.

Main Methods:

Examination and discussion of various RNA isolation techniques tailored for ctenophores.
Development of a convenient, inexpensive, and reproducible protocol for RNA-seq library preparation.
Application of the protocol to early embryonic stages (1-8 cells), tissues, and single organs.

Main Results:

Successful demonstration of the RNA isolation protocol on diverse ctenophore samples.
The protocol is effective for low-input amounts, including single cells or organs.
Applicability shown across multiple ctenophore species (Pleurobrachia bachei, Mnemiopsis, Bolinopsis, Beroe).

Conclusions:

The presented protocol offers a valuable tool for ctenophore transcriptome research.
It facilitates accessible and reproducible RNA-seq analysis in these marine invertebrates.
Enables deeper understanding of ctenophore biology at the molecular level.