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RinRK1 enhances NF receptors accumulation in nanodomain-like structures at root-hair tip
Ning Zhou1,2, Xiaolin Li1,2, Zhiqiong Zheng1
1National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Shanghai Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China.
Nature Communications
|April 26, 2024
Summary
Rhizobial Nod factor (NF) signaling requires NF receptors and the kinase RinRK1. This study reveals RinRK1 and Flotillin 1 (Flot1) organize NF receptor complexes at root hair tips, essential for symbiotic infection thread formation.
Area of Science:
- Plant-microbe interactions
- Molecular plant science
- Symbiotic signaling
Background:
- Legume-rhizobia symbioses are crucial for nitrogen fixation.
- Nod factor (NF) perception by NF receptors initiates symbiotic signaling.
- RinRK1 kinase is vital for infection thread (IT) formation but its mechanism is unclear.
Purpose of the Study:
- To elucidate the molecular mechanism of RinRK1 in NF signaling.
- To investigate the role of RinRK1 and Flotillin 1 (Flot1) in NF receptor localization and function.
- To understand the assembly of signaling complexes during symbiotic infection.
Main Methods:
- Co-immunoprecipitation assays to detect protein interactions.
- Confocal microscopy to visualize protein localization in root hairs.
- Genetic analysis of mutants deficient in RinRK1 and Flot1.
Main Results:
- RinRK1 interacts with NF receptors (NFR1, NFR5) and promotes their accumulation at root hair tips.
- Flotillin 1 (Flot1) associates with NF receptors and RinRK1.
- Both RinRK1 and Flot1 are essential for NF receptor complex assembly and localization upon NF stimulation.
Conclusions:
- RinRK1 and Flot1 facilitate the formation of localized plasma membrane signaling platforms.
- These platforms are critical for NF receptor complex assembly and subsequent symbiotic infection.
- The study reveals a novel mechanism for regulating symbiotic signaling in legumes.
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