Rhizobium leguminosarum genes involved in early stages of nodulation

Nodulation genes from Rhizobium leguminosarum have been subcloned and transferred to a strain of R. phaseoli with its symbiotic plasmid deleted (and therefore its nodulation and nitrogen fixation genes). Normal infection and nodule development occurred when these strains were added to the roots of Pisum sativum (peas) and Vicia hirsuta. The pea nodules were examined by electron microscopy; bacteroid forms were seen surrounded by peribacteroid membranes and using immuno-gold labelling it was shown that the nodule cells contained leghaemoglobin within the cytoplasm. By subcloning and analysing the nodulation region it was found that a small (3.2 X 10(3) bases) fragment of DNA contained three genes involved in root hair curling, the first observed step in the interaction between R. leguminosarum and the root hair cells of these legumes.