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CT-Visible Microspheres Enable Whole-Body In Vivo Tracking of Injectable Tissue Engineering Scaffolds.

Annalisa Bettini1,2, Peter Stephen Patrick1, Richard M Day2

  • 1Centre for Advanced Biomedical Imaging, Division of Medicine, University College London, London, WC1E 6DD, UK.

Advanced Healthcare Materials
|April 28, 2024
PubMed
Summary
This summary is machine-generated.

Injectable barium sulfate microspheres enable non-invasive tracking of tissue engineering scaffolds and transplanted cells. This innovation aids in optimizing regenerative therapies by confirming scaffold location, retention, and biodistribution.

Keywords:
computed tomographyinjectable scaffoldsmicrospheresmultimodal imagingtrackable

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Medical Imaging

Background:

  • Targeted delivery and retention are critical for implantable tissue-engineered products.
  • Non-invasive imaging is needed to track transplanted cells and scaffolds for optimizing regenerative therapies.

Purpose of the Study:

  • To develop an injectable, trackable tissue engineering scaffold for enhanced visualization and assessment of cell delivery and retention.
  • To evaluate the biocompatibility and tracking capabilities of barium sulfate-loaded microspheres for regenerative medicine applications.

Main Methods:

  • Microspheres were engineered with barium sulfate (BaSO4) as a computed tomography (CT) contrast agent.
  • Cellularization with GFP+ Luciferase+ mesenchymal stem cells and in vitro biocompatibility testing were performed.
  • In vivo tracking using 3D-bioluminescent imaging and micro-CT (µCT) co-registration in mice and minimally-invasive delivery in rats were conducted.

Main Results:

  • BaSO4-loaded microspheres demonstrated high X-ray absorption for whole-body tracking.
  • Cellularized microspheres remained viable in vivo for 14 days, showing good biocompatibility.
  • Co-registration of imaging modalities confirmed scaffold material and cell co-localization, and successful ultrasound-guided delivery was achieved.

Conclusions:

  • BaSO4-loaded microspheres serve as a novel tool for optimizing delivery techniques and tracking the persistence and distribution of implanted scaffold materials.
  • These trackable, cellularized microspheres hold potential as injectable combination products for cardiac regeneration and other tissue engineering applications.