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Protocol for recording neural activity evoked by electrical stimulation in mice using two-photon calcium imaging
Seungbin Park1, Megan Lipton1, Yujiao J Sun2
1Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA.
STAR Protocols
|April 28, 2024
Summary
This study details a protocol for recording neural activity in mice using electrical stimulation and two-photon calcium imaging. This method aids in understanding brain responses to electrical stimulation for neurological disorder treatments.
Area of Science:
- Neuroscience
- Biomedical Engineering
Background:
- Electrical stimulation is a promising clinical method for neurological disorders.
- Recording neural activity is crucial for understanding treatment mechanisms.
Purpose of the Study:
- To present a detailed protocol for recording neural activity evoked by electrical stimulation.
- To enable research into the effects of electrical stimulation on the nervous system.
Main Methods:
- Chronic implantation of head fixation bar, stimulating electrode, and glass imaging window in mice.
- Viral injections for targeted neural labeling.
- Two-photon calcium imaging during awake, head-fixed recordings.
Main Results:
- Successful recording of neural activity in response to electrical stimulation.
- Demonstration of a comprehensive protocol for in vivo neural imaging.
Conclusions:
- The presented protocol facilitates the study of neural circuits modulated by electrical stimulation.
- This technique supports the development of novel neuromodulation therapies for neurological conditions.
