Related Experiment Video
Updated: Jun 27, 2025
05:31
Isolation, Cryopreservation and Culture of Human Amnion Epithelial Cells for Clinical Applications
Published on: December 21, 2014
13.9K
Protocol for isolating amnion cells from human and non-human primate placenta for flow cytometry and transcriptomics.
Monica Cappelletti1, Daniel Short2, Marco Morselli3
1Divisions of Neonatology and Developmental Biology, David Geffen School of Medicine at the University of California Los Angeles, Los Angeles, CA 90095, USA.
STAR Protocols
|April 28, 2024
Summary
This study details a new protocol for isolating human and Rhesus macaque amnion cells. This method aids in understanding immune responses during pregnancy complications and developing therapies.
Area of Science:
- Reproductive biology
- Immunology
- Cell biology
Background:
- The amnion is a crucial fetal membrane at the feto-maternal interface during pregnancy.
- Understanding amnion cell function is vital for pregnancy health and disease research.
Purpose of the Study:
- To present a standardized protocol for isolating human and Rhesus macaque amnion cells.
- To enable further research into the immunological roles of amnion cells.
Main Methods:
- Tissue dissection of human and Rhesus macaque placentas.
- Cell isolation techniques for flow cytometry.
- RNA isolation for subsequent RNA sequencing.
Main Results:
- A reproducible protocol for obtaining viable amnion cells.
- Methodology for preparing cells for immunological and genetic analysis.
Conclusions:
- The developed protocol facilitates the study of amnion cell immunology.
- This research can inform the development of therapeutic strategies for intrauterine infections.
