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Updated: Jun 27, 2025

Microfluidics in Assessing Platelet Function

Microfluidics in Assessing Platelet Function

Published on: November 8, 2024

Lipidomic changes occurring in platelets during extended cold storage.

Sarah M Green^1,2, Matthew P Padula², Tyren M Dodgen³

¹Research & Development, Australian Red Cross Lifeblood, Alexandria, New South Wales, Australia.

Transfusion Medicine (Oxford, England)

|April 28, 2024

Summary

This summary is machine-generated.

Cold storage of platelets for 14 days increases procoagulant lipids phosphatidylserine (PS) and phosphatidylethanolamine (PE). However, bioactive lipids like lysophosphatidylcholine (LPC) remain stable, offering insights into platelet preservation.

Keywords:

lipid platelet transfusion

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Area of Science:

Blood banking and transfusion medicine
Lipidomics and mass spectrometry
Biochemistry and molecular biology

Background:

Platelet shelf-life is limited to 5-7 days at room temperature.
Cold storage is explored to extend platelet viability.
Understanding lipid profile changes is crucial for optimizing storage.

Purpose of the Study:

To characterize the lipid profile of platelets stored at room temperature versus cold conditions.
To assess changes in bioactive lipid mediators during platelet storage.
To evaluate the impact of cold storage on platelet lipid composition.

Main Methods:

Matched apheresis-derived platelets were stored at room temperature (20-24°C) or cold (2-6°C).
Lipidome analysis was performed using ultra-pressure liquid chromatography ion mobility quadrupole time of flight mass spectrometry (UPLC IMS QToF).
Bioactive lipid mediators were quantified by ELISA.

Main Results:

Total phospholipid and sphingolipid content remained similar over 14 days, irrespective of storage temperature.
Extended cold storage (14 days) increased procoagulant lipids phosphatidylserine (PS) and phosphatidylethanolamine (PE).
Sphingomyelin (SM) increased, ceramide decreased, and lysophosphatidylcholine (LPC) species were stable in cold-stored platelets. Hydroxyeicosatetraenoic acid (HETE) levels were lower in cold-stored platelet supernatant.

Conclusions:

Platelet lipid profiles are largely stable during 5-day storage, regardless of temperature.
Extended cold storage (14 days) alters the proportion of procoagulant lipids (PS, PE).
Lysophosphatidylcholine (LPC) and key bioactive lipids remain stable during extended cold storage.