Related Experiment Video
Updated: Jun 20, 2026
Single-molecule Super-resolution Imaging of Phosphatidylinositol 4,5-bisphosphate in the Plasma Membrane with Novel Fluorescent Probes
Published on: October 15, 2016
A Naphthalimide Based "Turn-ON" Probe for Wash-Free Imaging of Lipid-Droplet in Living Cells With an Excellent
Laurane Michel1, Philippe Durand1, Arnaud Chevalier1
1Université Paris-Saclay, CNRS, UPR 2301, Institut de Chimie des Substances Naturelles, Dpt Chemobiology, 91198, Gif-sur-Yvette, France.
Abstract:
The impacts of dimethylation of 4-Amino-1,8-Naphthalimide (ANI) on its photophysical properties are reported. The resulting 4-DiMe-ANI displays completely different fluorescence properties, conferring it ability to selectively label lipid droplets in living cells. A comprehensive photophysical study revealed that this selectivity arises from an Internal Charge Transfer favored in lipophilic media to the detriment of a non-emissive TICT in more polar media. This results in a very high "LDs/Cytosol" signal ratio, enabling LDs to be imaged with an excellent signal-to-noise ratio, and positioning its performance above that of the BODIPY 493/503 commonly used to image LDs.
More Related Videos
08:31Measuring Membrane Lipid Turnover with the pH-sensitive Fluorescent Lipid Analog ND6
Published on: July 29, 2021
08:55Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence TIRF Microscopy
Published on: February 17, 2023
