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Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

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The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
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Sanger Sequencing01:57

Sanger Sequencing

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Evolutionary Relationships through Genome Comparisons02:54

Evolutionary Relationships through Genome Comparisons

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Genome comparison is one of the excellent ways to interpret the evolutionary relationships between organisms. The basic principle of genome comparison is that if two species share a common feature, it is likely encoded by the DNA sequence conserved between both species. The advent of genome sequencing technologies in the late 20th century enabled scientists to understand the concept of conservation of domains between species and helped them to deduce evolutionary relationships across diverse...
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Maxam-Gilbert Sequencing01:05

Maxam-Gilbert Sequencing

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In the same year as the discovery of the Sanger sequencing method, another group of scientists, Allan Maxam and Walter Gilbert, demonstrated their chemical-cleavage method for DNA sequencing. The Maxam-Gilbert method relies on using different chemicals that can cleave the DNA sequence at specific sites, the separation of resulting DNA fragments of variable size using electrophoresis, and deciphering the DNA sequence from the resulting gel bands.
Challenges of the Maxam-Gilbert Method
The...
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Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Updated: Jun 27, 2025

Sequencing of mRNA from Whole Blood using Nanopore Sequencing
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DNA Sequencing Technologies and DNA Barcoding.

Anisha David1, J Deepa Arul Priya2, Akash Gautam3

  • 1Department of Botany, School of Life Sciences, St Joseph's University, Bengaluru, India.

Methods in Molecular Biology (Clifton, N.J.)
|April 29, 2024
PubMed
Summary
This summary is machine-generated.

DNA barcoding uses DNA segments for species identification. Advances like next-generation sequencing (NGS) and metabarcoding expand its use in biodiversity assessment and taxonomic classification.

Keywords:
BOLDDNA barcodingIllumina dye sequencingMetabarcodingNGSNanopore sequencingSanger sequencingiBOL

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Area of Science:

  • Genetics
  • Taxonomy
  • Bioinformatics

Background:

  • DNA barcoding utilizes standardized DNA regions for species identification.
  • Early methods relied on Sanger sequencing for taxonomic classification.
  • Next-generation sequencing (NGS) has broadened DNA barcoding applications.

Purpose of the Study:

  • To review the evolution and expanding applications of DNA barcoding.
  • To highlight the impact of technological advancements on species identification.
  • To discuss the future potential of DNA barcoding in taxonomy.

Main Methods:

  • Analysis of DNA barcode reference libraries.
  • Application of Sanger sequencing and next-generation sequencing (NGS).
  • Implementation of metabarcoding for complex sample analysis.

Main Results:

  • NGS has enabled wider applications including biomonitoring and biodiversity assessment.
  • Metabarcoding allows for high-throughput sequencing of bulk samples.
  • Technological progress enhances taxonomic classification accuracy.

Conclusions:

  • DNA barcoding, especially with NGS and metabarcoding, is crucial for modern taxonomy.
  • Future advancements promise significant progress in identifying and classifying species.
  • High-throughput sequencing techniques will revolutionize biodiversity studies.