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Related Concept Videos

DNA Isolation01:24

DNA Isolation

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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Updated: Jun 27, 2025

Use of a Filter Cartridge for Filtration of Water Samples and Extraction of Environmental DNA
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Environmental DNA Isolation, Validation, and Preservation Methods.

Seth W Cunningham1,2, Michael Tessler3,4,5, Jicayla Johnson-Rosemond6,7

  • 1Department of Biological Sciences, Fordham University, Bronx, NY, USA. scunningham@amnh.org.

Methods in Molecular Biology (Clifton, N.J.)
|April 29, 2024
PubMed
Summary
This summary is machine-generated.

This study details environmental DNA (eDNA) isolation, validation, and preservation methods. It provides a cost-effective protocol for extracting eDNA from diverse samples, emphasizing contamination control and proper storage for future research.

Keywords:
DNA quantificationDNA storageNucleic acid isolationSample preservationeDNA

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Area of Science:

  • Molecular Biology
  • Ecology
  • Environmental Science

Background:

  • Environmental DNA (eDNA) analysis relies on molecular techniques but requires specialized workflows.
  • Contamination control and appropriate negative controls are critical throughout the eDNA process, from collection to preservation.

Purpose of the Study:

  • To detail baseline methods for eDNA isolation, validation, and preservation.
  • To present a cost-effective, homebrew protocol optimized for eDNA extraction.

Main Methods:

  • Collection and preservation of diverse eDNA samples (soil, water, air, tissue).
  • Sample processing including inhibitor removal and physical disruption.
  • Standard DNA isolation (kit-based or phenol:chloroform:isoamyl [PCI]) and quantification (fluorometer).
  • Long-term storage strategies for sampled material and extracted DNA.

Main Results:

  • Established protocols for eDNA isolation, validation, and preservation.
  • Demonstrated a cost-effective homebrew extraction protocol.
  • Highlighted the importance of yield quantification before gene amplification.

Conclusions:

  • Standardized methods are crucial for reliable eDNA analysis.
  • Proper preservation ensures sample integrity and enables future research.
  • A cost-effective protocol can facilitate broader eDNA applications.