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Updated: Jun 27, 2025

Competitive Genomic Screens of Barcoded Yeast Libraries
Published on: August 11, 2011
Analysis of DNA Barcodes Using DNA Subway
Jason Williams1, Bruce Nash2, Cornel Ghiban2
1Cold Spring Harbor Laboratory DNA Learning Center, Cold Spring Harbor, NY, USA. williams@cshl.edu.
None:
DNA Subway makes bioinformatic analysis of DNA barcodes classroom friendly, eliminating the need for software installations or command line tools. Subway bundles research-grade bioinformatics software into workflows with an easy-to-use interface. This chapter covers DNA Subway's DNA barcoding analysis workflow (Blue Line) starting with one or more Sanger sequence reads. During analysis, users can view trace files and sequence quality, pair and align forward and reverse reads, create and trim consensus sequences, perform BLAST searches, select reference data, align multiple sequences, and compute phylogenetic trees. High-quality sequences with the required metadata can also be submitted as barcode sequences to NCBI GenBank.
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