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Updated: Jun 27, 2025

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography
Published on: March 12, 2017
Determination of the point resolution of high-resolution transmission electron microscope using the through-focus
Kurio Fukushima1, Yoshifumi Taniguchi2, Mitsuhide Matsushita3
1STEM Corporation, Tokyo 194-0215, Japan.
Abstract:
From the viewpoint of evaluating the instrumental performance of high-resolution electron microscopy (HREM), the Scherzer condition was investigated using information theory. As a result, the optimum defocus amount Δf can be expressed based on [Formula: see text] , and the formula [Formula: see text] is obtained. Furthermore, a procedure for measuring point resolution using the through-focus technique is developed, and a new method for determining the spherical aberration coefficient using the variance of Δf is introduced in the procedure.
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