Less interference fluorescence analytical strategy: Bridging substance-triggered ratiometric sensor with convenient...

Area of Science:

• Analytical Chemistry
• Nanomaterials Science
• Biomedical Sensing

Background:

• Existing fluorescence analysis methods suffer from high interference and limited application scope.
• There is a need for novel sensing systems with improved performance and wider applicability.

Purpose of the Study:

• To develop a novel ratiometric fluorescence sensing system for the simultaneous determination of berberine (BER) and daunorubicin (Dau).
• To overcome the limitations of traditional fluorescence analysis, including interference and narrow application range.

Main Methods:

• Construction of a dual-emission fluorescence sensing system using berberine (BER) and dihydrolipoic acid-protected silver nanoclusters (DHLA-AgNCs).
• Utilizing the conformational change of BER and fluorescence resonance energy transfer (FRET) for quantitative analysis.
• Monitoring changes in fluorescence intensity at 515 nm (BER) and 653 nm (DHLA-AgNCs) for analyte detection.

Main Results:

• The developed system exhibits dual emission peaks at 515 nm and 653 nm, enabling ratiometric detection.
• Sensitive determination of BER was achieved by monitoring the increase in fluorescence at 515 nm.
• Daunorubicin (Dau) was quantified by analyzing the alterations in dual emission peaks, leveraging BER's bridging effect and FRET.

Conclusions:

• The novel ratiometric fluorescence sensing system demonstrates strong anti-interference capabilities and low toxicity.
• This approach expands the application range of dual-emission detection systems, allowing analysis of compounds not directly measurable by silver nanoclusters.
• The study presents a new strategy for developing fluorescence sensing systems for challenging analytes.