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Updated: May 12, 2026

Detection of Bacteria Using Fluorogenic DNAzymes
Published on: May 28, 2012
Rapid and specific detection of thiabendazole: enzymatic digestion-enabled fluorescent aptasensor
Mohamed Amine Berkal1, Jean-Jacques Toulme2, Corinne Nardin3
1Universite de Pau Et Des Pays de L'Adour, E2S UPPA, CNRS, IPREM, Pau, France.
Abstract:
Thiabendazole, a widely used broad-spectrum fungicide in agriculture, poses risks to human health. To monitor its presence in water, we propose a fluorescent aptasensor utilizing Escherichia coli exonuclease I (Exo I). The findings demonstrate a linear correlation between thiabendazole concentrations and digestion percentage, with a detection limit (LOD) exceeding 1 µM and a determination coefficient (R2) of 0.959. This aptamer-based fluorescence spectroscopy detection system holds promise for a rapid, specific, and sensitive analysis of thiabendazole in environmental waters and food matrices.
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